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Forward Génétique écrans à l'aide pour identifier les macrophages<em> Toxoplasma gondii</em> gènes importants pour la résistance à l'IFN-γ-cellulaire dépendant immunité autonome
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JoVE 杂志 免疫与感染
Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
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Forward Génétique écrans à l'aide pour identifier les macrophages<em> Toxoplasma gondii</em> gènes importants pour la résistance à l'IFN-γ-cellulaire dépendant immunité autonome

10,923 Views

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11:21 min

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March 12, 2015

DOI:

10.3791/52556-v

DOI:
10.3791/52556-v

•

11:21 min
March 12, 2015

•

2 Views
, , , , , , ,
1Department of Microbiology and Immunology,New York Medical College

Summary

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Forward genetics is a powerful approach to identify genes in intracellular pathogens important for resistance to cell autonomous immunity. The current approach uses innate immune cells, specifically macrophages, to identify novel Toxoplasma gondii genes important for immune evasion.

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Walwyn, O., Skariah, S., Lynch, B., Kim, N., Ueda, Y., Vohora, N., Choe, J., Mordue, D. G. Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity. J. Vis. Exp. (97), e52556, doi:10.3791/52556 (2015).

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