Journal
/
免疫与感染
/
앞으로 유전학 식별하는 대 식세포를 사용하여 화면<em> 톡소 포자충</em> 유전자 중요 저항이하는 IFN-γ 의존 세포 자치 내성
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
Please note that all translations are automatically generated. Click here for the English version.

앞으로 유전학 식별하는 대 식세포를 사용하여 화면<em> 톡소 포자충</em> 유전자 중요 저항이하는 IFN-γ 의존 세포 자치 내성

10,923 Views

•

11:21 min

•

March 12, 2015

DOI:

10.3791/52556-v

DOI:
10.3791/52556-v

•

11:21 min
March 12, 2015

•

2 Views
, , , , , , ,
1Department of Microbiology and Immunology,New York Medical College

Summary

Automatically generated

Forward genetics is a powerful approach to identify genes in intracellular pathogens important for resistance to cell autonomous immunity. The current approach uses innate immune cells, specifically macrophages, to identify novel Toxoplasma gondii genes important for immune evasion.

Explore More Videos

Toxoplasma GondiiMacrophagesForward Genetics ScreenIFN-γCell Autonomous ImmunityParasite MutantsParasite GenesChronic InfectionTachyzoiteBradyzoite

Read Article

Cite this Article

Walwyn, O., Skariah, S., Lynch, B., Kim, N., Ueda, Y., Vohora, N., Choe, J., Mordue, D. G. Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity. J. Vis. Exp. (97), e52556, doi:10.3791/52556 (2015).

Download .ris file

Copy

Share Video

.

Copy