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마우스 해마 조직의 예 자궁 Electroporation에와 Organotypic 슬라이스 문화
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue

마우스 해마 조직의 예 자궁 Electroporation에와 Organotypic 슬라이스 문화

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09:17 min

March 04, 2015

DOI:

09:17 min
March 04, 2015

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Summary

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Here we present a protocol providing a tool to examine regulatory mechanisms of specific genes during hippocampal development. Employing ex utero electroporation and organotypic slice culture allows the up- and down-regulation of the expression of genes of interest in single cells and follow their fate during development.

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