Generatie van plasmide vectoren die-FLAG tagged eiwitten onder de verordening van de Mens elongatiefactor-1α Promoter Met behulp van Gibson Vergadering
Synthesis of custom plasmids is labor and time consuming. This protocol describes the use of Gibson assembly cloning to reduce the work and duration of custom DNA cloning procedure. The protocol described also produces reliable tagged protein constructs for mammalian expression at similar cost to the traditional cut-and-paste DNA cloning.