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质粒载体产生表达FLAG标记的蛋白质在人延伸因子1α启动子的使用吉布森大会规则
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

质粒载体产生表达FLAG标记的蛋白质在人延伸因子1α启动子的使用吉布森大会规则

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10:18 min

February 09, 2015

DOI:

10:18 min
February 09, 2015

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Summary

Automatically generated

Synthesis of custom plasmids is labor and time consuming. This protocol describes the use of Gibson assembly cloning to reduce the work and duration of custom DNA cloning procedure. The protocol described also produces reliable tagged protein constructs for mammalian expression at similar cost to the traditional cut-and-paste DNA cloning.

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