This content is Free Access.
Erzeugung von Plasmid-Vektoren, die FLAG-markierten Proteine Nach der Verordnung der Menschen Elongation Factor-1α Promoter Mit Gibson Assembly
36,244 Views
•
10:18 min
•
February 09, 2015
DOI:
Summary
Automatically generated
Synthesis of custom plasmids is labor and time consuming. This protocol describes the use of Gibson assembly cloning to reduce the work and duration of custom DNA cloning procedure. The protocol described also produces reliable tagged protein constructs for mammalian expression at similar cost to the traditional cut-and-paste DNA cloning.
Read Article
Cite this Article
Grozdanov, P. N., MacDonald, C. C. Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly. J. Vis. Exp. (96), e52235, doi:10.3791/52235 (2015).
Copy