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Immunology and Infection

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JoVE Journal Immunology and Infection
Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells
 

Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells

Article DOI: 10.3791/52119-v • 08:27 min • October 14th, 2014
October 14th, 2014 •
1, 1, 1, 2, 2, 1
1Department of Biological Sciences, Hunter College and Graduate Center, City University of New York, 2Sunnybrook Research Institute, Department of Immunology, University of Toronto

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Automatic Translation

Mouse embryonic stem cells can be differentiated to T cells in vitro using the OP9-DL1 co-culture system. Success in this procedure requires careful attention to reagent/cell maintenance, and key technique sensitive steps. Here we discuss these critical parameters and provide a detailed protocol to encourage adoption of this technology.

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T Cells In Vitro Mouse Embryonic Stem Cells OP9/OP9-DL1 Co-culture System Differentiated Blood Cell Types Embryonic And Hematopoietic Progenitors Genetic Cellular And Molecular Questions Hematopoiesis Therapeutic Applications Reagent And Cell Culture Maintenance Protocol Technique Sensitive Steps Differentiation Of T Lymphocytes Reproducibly Robust Cellular Differentiation
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