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Slice It Hot: aiguë cerveau adulte trancher dans physiologique Température
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature
DOI:

08:46 min

October 30, 2014

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Chapters

  • 00:05Title
  • 01:17Setting Up for the Slicing Procedure
  • 02:53Dissecting the Brain
  • 04:52Brain Slicing
  • 07:09Results: Patch-clamp Recordings from Cerebellar Neurons
  • 08:25Conclusion

Summary

自动翻译

In this paper we show a method for preparing acute brain slices in physiological temperature, using a conventional physiological solution without special modifications for the cutting (such as adding sucrose) and without intracardial perfusion of the animal before slice preparation.

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