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Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

DOI:
10.3791/52038-v

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10:29 min

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October 08, 2014

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, ,
1Institute of Neurobiology,Heinrich Heine University Düsseldorf

Chapters

  • 00:05Title
  • 01:56Dissection of Tissue
  • 03:05Preparation of Hardware
  • 05:03Whole-cell Patch-clamping
  • 06:11Multi-photon Imaging and Stimulation
  • 08:05Results: Sodium Signals and Synaptic Currents Induced by Flash Photolysis of Caged Glutamate
  • 09:43Conclusion

Summary

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自动翻译

We describe the combination of focal UV-induced photo-activation of neuro-active compounds with whole-cell patch-clamp and multi-photon imaging of intracellular sodium transients in dendrites and spines of hippocampal neurons in acute tissue slices of the mouse brain.

Tags

Keywords: Multi-photon ImagingSodium ImagingUV-mediated UncagingGlutamate UncagingCA1 Pyramidal NeuronsWhole-cell Patch-clampSBFIMNI-glutamateIonotropic Glutamate ReceptorsDendritic SpinesHippocampus

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