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Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

13,622 Views

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10:29 min

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October 08, 2014

DOI:

10.3791/52038-v

DOI:
10.3791/52038-v

•

10:29 min
October 08, 2014

•

11 Views
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1Institute of Neurobiology,Heinrich Heine University Düsseldorf

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Multi-photon ImagingSodium ImagingUV-mediated UncagingGlutamate UncagingCA1 Pyramidal NeuronsWhole-cell Patch-clampSBFIMNI-glutamateIonotropic Glutamate ReceptorsDendritic SpinesHippocampus

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Kleinhans, C., Kafitz, K. W., Rose, C. R. Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons. J. Vis. Exp. (92), e52038, doi:10.3791/52038 (2014).

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