Journal
/
/
Imaging intracelular de Ca<sup> 2 +</sup> Las señales en estriatal astrocitos de ratones adultos a través de indicadores de calcio codificados genéticamente-
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators

Imaging intracelular de Ca<sup> 2 +</sup> Las señales en estriatal astrocitos de ratones adultos a través de indicadores de calcio codificados genéticamente-

14,642 Views

12:19 min

November 19, 2014

DOI:

12:19 min
November 19, 2014

22 Views
, , ,

Summary

Automatically generated

The properties and functions of astrocyte intracellular Ca2+ signals in the striatum remain incompletely explored. We describe methods to express genetically encoded calcium indicators in striatal astrocytes using adeno-associated viruses of serotype 2/5 (AAV2/5), as well as procedures to reliably image Ca2+ signals within striatal astrocytes in situ.

Read Article