Imaging intracelular de Ca<sup> 2 +</sup> Las señales en estriatal astrocitos de ratones adultos a través de indicadores de calcio codificados genéticamente-
Imaging intracelular de Ca<sup> 2 +</sup> Las señales en estriatal astrocitos de ratones adultos a través de indicadores de calcio codificados genéticamente-
The properties and functions of astrocyte intracellular Ca2+ signals in the striatum remain incompletely explored. We describe methods to express genetically encoded calcium indicators in striatal astrocytes using adeno-associated viruses of serotype 2/5 (AAV2/5), as well as procedures to reliably image Ca2+ signals within striatal astrocytes in situ.
Jiang, R., Haustein, M. D., Sofroniew, M. V., Khakh, B. S. Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators. J. Vis. Exp. (93), e51972, doi:10.3791/51972 (2014).