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内皮细胞，细胞连接实时成像过程中中性粒细胞轮回在生理流动

JoVE 杂志
免疫与感染

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JoVE 杂志免疫与感染

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

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内皮细胞，细胞连接实时成像过程中中性粒细胞轮回在生理流动

DOI:

10.3791/51766-v

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11:26 min

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August 14, 2014

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Jeffrey Kroon*¹, Anna E. Daniel*¹, Mark Hoogenboezem, Jaap D. van Buul

¹Department of Molecular Cell Biology,Sanquin Research and Landsteiner Laboratory, AMC at University of Amsterdam

Chapters

00:05Title
01:59Plating and Maintenance of Human Umbilical Vein Endothelial Cells
04:00Polymorphonuclear Leukocyte Isolation Using Percoll Gradients
06:36Labeling of Endothelial Junctional VE-cadherin and PECAM-1 and Performing the PMN TEM Assay Under Flow
09:24Results: Visualization of the Dynamics of VE-cadherin and PECAM-1 During Neutrophil TEM
10:59Conclusion

Summary

自动翻译

白细胞交叉使用旁或跨细胞途径的单层内皮细胞。我们开发了一个简单的试验，以遵循内源交界的分布VE-cadherin和PECAM-1白细胞跨内皮迁移过程中的生理流动下两路轮回区分。

Tags

Endothelial Cell-cell Junctions Neutrophil Transmigration Physiological Flow Leukocyte Transendothelial Migration Paracellular Route Transcellular Route VE-cadherin PECAM-1 In Vitro Assay Fluorescent Antibodies Electrical Cell-substrate Impedance Sensing FRAP Measurements

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