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1Department of Medicine, Division of Pulmonary and Critical Care Medicine,Brigham and Women’s Hospital, 2Department of Medicine, Renal Division,Brigham and Women’s Hospital, Harvard Medical School, 3First Department of Critical Care Medicine and Pulmonary Services,University of Athens Medical School, Evangelismos Hospital, Athens, Greece
Chapters
- 00:05Title
- 01:45Cecal Ligation and Puncture
- 03:44Tissue harvest and Fixation
- 05:06Results: Autophagy in Mice after Cecal Ligation and Puncture
- 06:05Conclusion
Sepsi sperimentale può essere indotta in topi usando la legatura del cieco e puntura metodo (CLP). Attuali protocolli per valutare autofagia in vivo nel contesto della sepsi CLP-indotta sono presentati qui: Un protocollo per misurare autofagia utilizzando topi (GFP)-LC3, e un protocollo per misurare formazione dell'autofagosoma mediante microscopia elettronica.
