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从人类胸腺髓样树突状细胞和上皮细胞的分离
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
DOI:

09:07 min

September 19, 2013

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Chapters

  • 00:05Title
  • 01:11Preparation of the Tissue
  • 02:45Typical Enzymatic Tissue Digestion to Obtain a Single Cell Suspension
  • 04:35Enrichment of Low Density Fraction (LDF) of Cells Using Percoll Density Separation
  • 06:00Results: Representative Myeloid DC and TEC Populations Isolated from the Human Thymus
  • 08:06Conclusion

Summary

自动翻译

这个协议的细节的方法来分离抗原通过不同的步骤的组织随后在单细胞悬浮液的密度离心和感兴趣的细胞群的最后磁和/或FACS分选的酶消化从人胸腺呈递细胞。

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