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Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates
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JoVE 杂志 免疫与感染
Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates

Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates

DOI:
10.3791/50409-v

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05:53 min

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July 06, 2013

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, , ,
1ImmunoRhumatologie Moléculaire, INSERM UMR_S 1109, Centre de Recherche d’Immunologie et d’Hématologie,Université de Strasbourg

Chapters

  • 00:05Title
  • 01:16Preparation of Viral Suspension
  • 02:14Injection of Neonates
  • 03:25In vivo Imaging
  • 05:00Results: Viral Expression Over Time
  • 05:32Conclusion

Summary

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Human Cytomegalovirus (HCMV) infection of neonates represents an important cause of mental retardation, yet the molecular events leading to virus-induced pathogenesis are still poorly understood. To investigate the dynamics of brain infection, we adapted whole-animal in vivo imaging to perform time-course analysis of neonates infected with a luciferase-recombinant virus.

Tags

In Vivo ImagingMouse CytomegalovirusMCMVNeonatal InfectionCentral Nervous SystemCNSViral DisseminationLuciferase ReporterTime-course Analysis

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