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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
 

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Article DOI: 10.3791/50058-v • 09:28 min • February 2nd, 2013
February 2nd, 2013 •
1, 2, 1, 2, 1
1Department of Chemical and Biomolecular Engineering, University of Houston, 2Division of Pediatrics, Research Unit 907, University of Texas MD Anderson Cancer Center

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We describe a single-cell high-throughput assay to measure cytotoxicity of T cells when incubated with tumor target cells. This method employs a dense, elastomeric array of sub-nanoliter wells (~100,000 wells/array) to spatially confine the T cells and target cells at defined ratios and is coupled to fluorescence microscopy to monitor effector-target conjugation and subsequent apoptosis.

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Quantitative High-throughput Single-cell Cytotoxicity Assay T Cells Cancer Immunotherapy Adoptive Cell Therapy Chimeric Antigen Receptor (CAR) Clinical Trials Non-MHC Restricted Antigens Apoptosis Host Toxicity Clinical Benefit Molecular Features Functional Features T-cell Preparations In Vitro Assays Cytotoxicity Cytokine Secretion Flow-cytometry Based Assays
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