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1Protein Crystallization Lab,Emerald Bio, 2Molecular Biology Lab,Emerald Bio, 3Scientific Sales Representative,Emerald Bio, 4Group Leader II,Emerald Bio, 5Group Leader I,Emerald Bio, 6Chair of Advisory Board,Emerald Bio, 7Director of Multi-Target Services,Emerald Bio, 8Senior Project Leader,Emerald Bio, 9Project Leader II & SSGCID Site Manager,Emerald Bio
Chapters
- 00:05Title
- 02:20Construct Design
- 04:07Polymerase Incomplete Primer Extension (PIPE) Cloning
- 06:14Expression Optimization
- 08:21Large Scale Fermentation
- 09:40Protein Purification – Cell Lysis
- 10:54Protein Purification – Affinity Chromatography
- 13:04Protein Purification – Affinity Tag Cleavage
- 14:47Protein Purification – Size-exclusion Chromatography (SEC)
- 15:42Protein Crystallization
- 17:03Crystal Harvesting
- 18:15Crystal Screening/Data Collection
- 19:27Results: 3D Structure Determination of Influenza Virus PB2
- 21:05Conclusion
Structure-based drug design spielt eine wichtige Rolle in der Medikamentenentwicklung. Verfolgen mehrere Ziele parallel erhöht die Chance auf Erfolg für Blei Entdeckung. Der folgende Artikel zeigt, wie die Seattle Structural Genomics Center for Infectious Disease eine Multi-Target-Ansatz für die Gen-to-Bestimmung der Struktur des Influenza-A-Untereinheit PB2 nutzt.
Tags
Multi-targetParallel ProcessingGene-to-structure DeterminationInfluenza Polymerase PB2 SubunitPandemic OutbreaksInfluenza Virus InfectionPoint MutationsVirulence FactorAntiviral Drug TargetStructural Genomics ProgramNational Institute Of Allergy And Infectious Disease (NIAID)Emerald BioSeattle Structural Genomics Center For Infectious Disease (SSGCID)Three-dimensional Protein StructuresNIAID Category A-C PathogensStructure-based Drug DesignDrug Development
