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Isolering og dyrkning af neurale kamceller fra embryonale Murin Neural Tube
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
DOI:

12:48 min

June 02, 2012

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Chapters

  • 00:05Title
  • 01:14Introduction
  • 01:36Prepare Plates
  • 02:18Preparation of Self Renewal and Wash Media
  • 03:06Preparation of Collagenase/Dispase
  • 04:22Isolating Vagal and Trunk Neural Tube
  • 08:21Removal of Non-Neural Ectoderm and Mesoderm
  • 11:06Removal of the Neural Tube
  • 11:46Representative Results
  • 12:35Conclusions

Summary

自动翻译

Isolering af embryoniske crista neuralis fra neuralrøret letter anvendelsen af<em> In vitro</em> Metoder til at studere migration, selvfornyelse, og multipotency af neural crest.

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