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Uno screening genetico per isolare Toxoplasma gondii Host-cell Egress Mutants
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February 08, 2012
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Forward genetica è un metodo efficace per svelare il livello molecolare di come<em> Toxoplasma</em> Egresses dalla sua cellula ospite. I protocolli sono fornite mutagenizzare chimicamente parassiti, per arricchire mutanti con difetti in uscita indotta, e convalidare il fenotipo di mutanti clonati.
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Genetic ScreenToxoplasma GondiiHost-cell Egress MutantsObligate Intracellular ParasiteLytic Replication CycleActive InvasionReplication Inside Host CellActive EgressMolecular LevelSignaling PathwaysPharmacological AgentsTriggers Of EgressIntracellular Ca2+Candidate Gene ApproachPlant Like Calcium Dependent Protein Kinase (CDPK)(chemical) Genetic ApproachesScreen For Mutant ParasitesDefect In Host Cell EgressChemical MutagenesisN-ethyl-N-nitrosourea (ENU)
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Coleman, B. I., Gubbels, M. A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants. J. Vis. Exp. (60), e3807, doi:10.3791/3807 (2012).
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