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Profiling Proteome Redox tiol Usando Isótopos Espectrometria de Massa Tagging
JoVE 杂志
生物学
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JoVE 杂志 生物学
Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Profiling Proteome Redox tiol Usando Isótopos Espectrometria de Massa Tagging

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12:07 min

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March 24, 2012

DOI:

10.3791/3766-v

DOI:
10.3791/3766-v

•

12:07 min
March 24, 2012

•

2 Views
, , ,
1Plant Molecular and Cellular Biology Program,University of Florida , 2Department of Biology,University of Florida , 3Interdisciplinary Center for Biotechnology Research,University of Florida , 4Genetics Institute,University of Florida

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Nível de espécies reativas de oxigênio é elevado quando as células encontrar condições de estresse. Aqui mostramos o exemplo de 3'-3 'diaminobenzidina coloração, bem como cysTMT rotulagem e espectrometria de massa para traçar o perfil do proteoma redox em<em> Pseudomonas syringae</em> Tratada folhas de tomateiro.

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Cite this Article

Parker, J., Zhu, N., Zhu, M., Chen, S. Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry. J. Vis. Exp. (61), e3766, doi:10.3791/3766 (2012).

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