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Chapters
- 00:05Title
- 02:07Inoculation of Tomato
- 03:13Protein Extraction
- 04:21Sample Preparation and Peptide Labeling with cysTMT
- 06:07Removal of Non-reacted Tag Sample Fractionation
- 07:44Sample Enrichment of cysTMT-labeled Peptides
- 08:43Mass Spectrometry Analysis
- 10:02CysTMT Results: Profiling Thiol Redox Proteome of Pseudomonas syringae in Tomato
- 11:11Conclusion
Reaktive Sauerstoffspezies Niveau angehoben wird, wenn Zellen begegnen Stressbedingungen. Hier zeigen wir das Beispiel von 3'-3 'Diaminobenzidin-Färbung sowie cysTMT Kennzeichnung und Massenspektrometrie, um die Redox-Proteom im Profil<em> Pseudomonas syringae</em> Behandelt Tomatenblättern.
Tags
Thiol Redox ProteomeIsotope Tagging Mass SpectrometryPseudomonas Syringae Pv. TomatoBacterial Speck DiseaseSolanum LycopersicumBrassica SpeciesArabidopsis ThalianaReactive Oxygen Species (ROS)Hydrogen PeroxideDAB StainingRedox Status Of ProteinsCysteine OxidationTandem Mass Tag (TMT) ReagentsCysteine-reactive TMT (cysTMT) Tags
