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JoVE Journal Neuroscience
Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers
 

Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers

Article DOI: 10.3791/3755-v • 14:06 min • June 26th, 2012
June 26th, 2012 •
1,2, 3, 2,4, 3
1Department of Neuroscience, Johns Hopkins University, 2Garvan Institute of Medical Research, 3School of Biological Sciences, Washington State University, 4Department of Otolaryngology-HNS, Johns Hopkins University

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Electrophysiological characterization of neuronal responses is important for understanding brain function and for guiding the placement of dyes for pathway tracing. However, many studies are performed in anesthetized animals. To understand brain function without anesthetics, we developed a method to record neuronal response properties and inject dyes in awake mouse.

Tags

Awake Mouse Recording Neural Responses Injecting Tracers Anesthesia Brainstem Neurons Fundamental Response Properties Decerebrate Preparation Electrode Arrays Chronic Recording Awake And Behaving Animals Tracer Dyes Head Restraint Technique
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