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定量成像小样本人体血液中单核细胞和树突状细胞系特定的Toll样受体介导的信号
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07:58 min
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April 16, 2012
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我们描述ImageStream技术的使用（<a href="http://www.amnis.com/" target="_blank"> www.amnis.com</a>），它结合了定量流式细胞仪同时高分辨率数字成像，量化定义良好的病人同伙的主要免疫细胞的细胞机制。我们的研究提供了一个蓝图转化调查量化谱系在小样本的具体细胞的反应，受同伙。
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Quantitative ImagingLineage-specificToll-like Receptor-mediated SignalingMonocytesDendritic CellsSmall SamplesHuman BloodImmune StatusInfectionDisease SeverityOutcomeAgingViral InfectionsBacterial InfectionsVaccinesHumoral Immune ResponsesCell-mediated Immune ResponsesToll-like Receptors (TLRs)Innate Immune SystemAntimicrobial Host Defense ResponsesPeripheral Blood MonocytesElderly DonorsDendritic Cells (DCs)Antigen-presenting CellsInnate ImmunityAdaptive ImmunityDysregulation Of TLR3MacrophagesIFN ProductionWest Nile Virus InfectionImmunosenescenceTherapeutic Intervention
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Cite this Article
Qian, F., Montgomery, R. R. Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood. J. Vis. Exp. (62), e3741, doi:10.3791/3741 (2012).
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