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Chapters
- 00:05Title
- 02:22Isolation of Human Peripheral Blood Mononuclear Cells (PBMCs) from Buffy Coat
- 03:58Magnetic Negative Selection of T Cells from PBMCs using EasySep Enrichment Kit
- 06:16Cell Culture Conditions to Induce Regulatory T Cells
- 07:11Fluorescence-activated Cell Sorting of Three Populations of T Cells
- 08:28Suppression Assay
- 10:32Representative Sorting and Suppression Assay Results
- 13:14Conclusion
Tags
Induced Regulatory T CellsPrimary Human Naive T CellsMemory T CellsImmunosuppressive CD4+ Regulatory T CellsPeripheral ToleranceImmunologic HomeostasisSelf AntigensCommensal AntigensTreg BiologyTherapeutic Injection Of TregsMurine ModelsColitisType I DiabetesRheumatoid ArthritisGraft Versus Host DiseaseEx Vivo ExpansionPeripheral Blood Mononuclear CellsCD4+ T CellsIn Vitro Cell CultureFluorescence Activated Cell Sorting (FACS)Forkhead Box P3 (FoxP3)Activation-induced Transcription Factor
