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Induktion af graft-versus-host sygdom og<em> In Vivo</em> T Cell Monitoring Brug en MHC-matchet murin model
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JoVE 杂志 免疫与感染
Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model
DOI:

10:29 min

August 29, 2012

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Chapters

  • 00:05Title
  • 01:10Lethal Irradiation and Splenocyte Preparation
  • 03:12Bone Marrow Preparation and Removal
  • 05:16CD3 Depletion
  • 06:08L2G85.B6 (LUX+) CD8+ T Cell Purification and Injection Preparation
  • 07:48Bioluminescent Imaging
  • 08:12Results
  • 10:12Conclusion

Summary

自动翻译

Murin knoglemarvstransplantation er en meget anvendt teknik til at undersøge immunologiske mekanismer for transplantat-versus-host-sygdom hos mennesker. Muligheden for at overvåge T-celle trafficking mønstre<em> In vivo</em> Giver mulighed for detaljeret analyse af udviklingen og bevarelsen af ​​T-celle responser under graft-versus-host sygdom.

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