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Immunology and Infection

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JoVE Journal Immunology and Infection
High-throughput Detection Method for Influenza Virus
 

High-throughput Detection Method for Influenza Virus

Article DOI: 10.3791/3623-v • 10:05 min • February 4th, 2012
February 4th, 2012 •
1, 1, 2, 3, 4, 4, 1,5, 1,6
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, 2Department of Microbiology, Mount Sinai School of Medicine, 3Laboratory of Molecular Genetics, Blood Research Institute, 4City of Milwaukee Health Department Laboratory, 5Division of Hematology-Oncology/BMT, Children's Hospital of Wisconsin, Medical College of Wisconsin, 6Division of Hematology and Oncology, Dept Medicine, Medical College of Wisconsin

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This method describes the use of Infrared dye based imaging system for detection of H1N1 in bronchioalveolar lavage (BAL) fluid of infected mice at a high sensitivity. This methodology can be performed in a 96- or 384-well plate, requires <10 μl volume of test material and has the potential for concurrent screening of multiple pathogens.

Tags

High-throughput Detection Method Influenza Virus Precise Diagnosis Infecting Strain Rapid Screening Clinical Samples Pandemic Infections Serologic Testing Polymerase Chain Reaction Direct Specimen Immunofluorescence Cell Culture Novel Diagnostic Technique Live Influenza Viruses Mouse-adapted Human A/PR/8/34 (PR8 H1N1) Virus MDCK Cells Viral Proteins Anti-M2 Antibody Hemagglutinin 1 Diagnostic Assays IR-dye-conjugated Secondary Antibodies Antigen-specific Fluorescence Intensity LI-COR Odyssey-based IR Scanner
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