Journal
/
神经科学
/
Påvisning af Microregional Hypoxia i mus hjernebarken ved to-foton Imaging af endogent NADH Fluorescens
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

Påvisning af Microregional Hypoxia i mus hjernebarken ved to-foton Imaging af endogent NADH Fluorescens

DOI:
10.3791/3466-v

•

12:03 min

•

February 21, 2012

•

, , , , ,
1Department of Microbiology and Immunology,University of Rochester Medical Center, 2Center for Neural Development and Disease,University of Rochester Medical Center, 3Deptartment of Neurology, Center for Neural Development and Disease,University of Rochester Medical Center

Chapters

  • 00:05Title
  • 01:42Preparing the Animal for IMaging
  • 02:56Preparing the Open Skull Cranial Window
  • 06:40Intravenous Injection and Blood Oxygenation Monitoring
  • 07:58Two-photon Imaging
  • 09:30Representative Hypoxia Measurement Results
  • 11:14Conclusion

Summary

自动翻译

自动翻译

Her beskriver vi en fremgangsmåde til direkte at visualisere microregional vævshypoxi i mus cortex<em> In vivo</em>. Det er baseret på samtidig to-foton-billeddannelse af nicotinamid-adenin-dinucleotid (NADH) og corticale mikrocirkulation. Denne fremgangsmåde er nyttig til høj opløsning analyse af væv oxygentilførsel.

Tags

DetectionMicroregional HypoxiaMouse Cerebral CortexTwo-photon ImagingEndogenous NADH FluorescenceOxygen SupplyBlood FlowVisualized Experimental ProtocolMethodological ProtocolPerivascular Oxygen GradientsNon-linear RelationshipNicotinamide Adenine Dinucleotide (NADH)Fluorescence IntensityOxygen Partial PressureTwo-photon ExcitationTexas-Red DextranAdvantagesStandard Two-photon In Vivo Imaging EquipmentContinuous MonitoringDepth ResolutionVulnerable Watershed AreasDecline In Vascular Oxygen SupplyMicroregional Cortical Oxygenation

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/3466?article_type=v

In vivo imaging af Mouse Spinal Cord ved hjælp af to-foton mikroskopi
check_url/cn/3466?article_type=v

In vivo to-foton billedbehandling af oplevelsesbaseret afhængige molekylære ændringer i kortikal neuroner
check_url/cn/3466?article_type=v

Funktionel Imaging af Auditory Cortex i voksne katte ved hjælp af High-felt fMRI
check_url/cn/3466?article_type=v

To-foton<em> In vivo</em> Imaging af dendritiske spidser i Mouse Cortex Ved hjælp af en tyndere-kranium Forberedelse
check_url/cn/3466?article_type=v

To-foton Imaging for Cellulær Dynamics i Mouse Spinal Cord
check_url/cn/3466?article_type=v

Samtidig To-foton<em> In vivo</em> Imaging af synaptisk indgange og Postsynaptiske Mål i Mouse retrospenialis Cortex
check_url/cn/3466?article_type=v

Kvantificering af cerebral vaskulær arkitektur ved hjælp af to-foton mikroskopi i en musemodel af HIV-induceret Neuroinflammation
check_url/cn/3466?article_type=v

To-foton Calcium Imaging i Neuronal dendritter i hjernen skiver
check_url/cn/3466?article_type=v

In Vivo To-foton billeddannelse af kortikale neuroner i Neonatal mus
check_url/cn/3466?article_type=v

Transpupillær to-foton In vivo Billeddannelse af musens nethinde

Read Article