This content is Free Access.
Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart
26,680 Views
•
08:13 min
•
September 13, 2011
DOI:
Summary
Automatically generated
This paper details the dissection procedure, instrumental setup, and experimental conditions during optical mapping of transmembrane potential (Vm) and intracellular calcium transient (CaT) in intact isolated Langendorff perfused mouse hearts.
Explore More Videos
Optical MappingAction PotentialsCalcium TransientsMouse HeartCardiovascular StudiesFluorescence ImagingGenetic EngineeringPhysiological PhenotypingTransmembrane Potential (Vm)Excitation-contraction CouplingLangendorff Perfused Mouse HeartsHeart FailureArrhythmiasMetabolic DiseaseHeart DiseasesActivation VisualizationConduction VelocityAction Potential DurationInstrumentation SetupExperimental ConditionsSpatio-temporal ResolutionCMOS Imaging Technology
Read Article
Cite this Article
Lang, D., Sulkin, M., Lou, Q., Efimov, I. R. Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart. J. Vis. Exp. (55), e3275, doi:10.3791/3275 (2011).
Copy