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Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart
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Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart
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September 13, 2011
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This paper details the dissection procedure, instrumental setup, and experimental conditions during optical mapping of transmembrane potential (Vm) and intracellular calcium transient (CaT) in intact isolated Langendorff perfused mouse hearts.
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Optical MappingAction PotentialsCalcium TransientsMouse HeartCardiovascular StudiesFluorescence ImagingGenetic EngineeringPhysiological PhenotypingTransmembrane Potential (Vm)Excitation-contraction CouplingLangendorff Perfused Mouse HeartsHeart FailureArrhythmiasMetabolic DiseaseHeart DiseasesActivation VisualizationConduction VelocityAction Potential DurationInstrumentation SetupExperimental ConditionsSpatio-temporal ResolutionCMOS Imaging Technology
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Lang, D., Sulkin, M., Lou, Q., Efimov, I. R. Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart. J. Vis. Exp. (55), e3275, doi:10.3791/3275 (2011).
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