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1Diabetes and Obesity Research Center,Sanford-Burnham Medical Research Institute at Lake Nona, 2Department of Molecular Physiology and Biophysics,Vanderbilt University School of Medicine, 3Vanderbilt Mouse Metabolic Phenotyping Center,Vanderbilt University School of Medicine, 4Department of Pediatrics and Cellular and Integrative Physiology,Indiana University School of Medicine
Chapters
- 00:05Title
- 01:23Surgery
- 05:21Hyperinsulinemic-euglycemic Clamp
- 09:09Evaluation of Insulin Action in Chow-fed vs. High Fat-fed Obese Mice using the Hyperinsulinemic-euglycemic Clamp
- 10:34Conclusion
O grampo hiperinsulinêmico euglicêmico-, ou clamp de insulina, é o padrão ouro para avaliar a ação da insulina<em> In vivo</em>. Um método para a realização de grampos de insulina em ratos é descrito. Isto inclui um método para cateterização arterial que permite experimentos a serem realizados no consciente, camundongos sem impedimentos, com o mínimo de stress.
Tags
Hyperinsulinemic-euglycemic ClampsInsulin ActionType 2 DiabetesInsulin InfusionGlucose InfusionGlucose Infusion Rate (GIR)Whole-body Insulin ActionIsotopic 2[14C]deoxyglucose[3-3H]glucoseEndogenous Glucose Appearance (endoRa)Hepatic Glucose ProductionWhole-body Glucose Disappearance (Rd)Genetic Mouse Models Of Metabolic DiseaseDiabetes ResearchInsulin Clamp Performance
