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单细胞在斑马鱼的命运映射
JoVE 杂志
生物学
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JoVE 杂志 生物学
Single Cell Fate Mapping in Zebrafish
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单细胞在斑马鱼的命运映射

DOI:
10.3791/3172-v

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07:53 min

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October 05, 2011

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, ,
1Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center, 2Division of Hematology/Oncology,Cincinnati Children’s Hospital Medical Center

Chapters

  • 00:05Title
  • 01:25Two-photon Activation of Caged Fluorescein-dextran
  • 05:03Immunodetection of Uncaged Fluorescein-dextran
  • 06:04Photoactivation and Immunodetection of Caged Fluorescein-dextran
  • 07:11Conclusion

Summary

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自动翻译

一种方法是描述photoactivate包含使用双光子吸收钛笼中的荧光蛋白单细胞：蓝宝石飞秒激光振荡器。命运地图的光敏细胞，免疫组化法是使用。这种技术可以应用到任何类型的细胞。

Tags

Single Cell Fate MappingZebrafish发育生物学Fate MappingLineage TracingUndifferentiated Precursor CellsPhotoactivatable ProteinsFluorescence SpectrumUV ExcitationCell Tracing ProbesLong-term Cell TrackingCaged Fluorescein-dextranEmbryo Model SystemsUncaging Fluorescein-dextranSpatial ResolutionPhotoactivationNear-infrared Laser PulsesTitanium Sapphire Femtosecond LaserTwo-photon Confocal Microscopes

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