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Chapters
- 00:05Title
- 03:34Generating Conditioned Medium (CM) For the Colony Assay
- 05:13Obtaining a Single Cell Suspension of Pancreatic-like Progenitors by Fluorescence Activated Cell Sorting
- 07:55Plating Sorted Cells to Semi-solid Culture
- 10:49Observing Colony Formation under the Light Microscope
- 11:47Ngn3-EGFP+ Derived Colonies Express Insulin
- 13:21Conclusion
En tre-dimensjonal clonogenic analysen som gjør at pankreas-lignende stamfedre å differensiere i insulin-uttrykke kolonier er beskrevet. Denne metoden tar fordel av semi-faste medier inneholder methylcellulose, Matrigel og vekstfaktorer, som enkelt stamfedre spre seg og differensiere<em> In vitro</em>, Tillater kvantifisering av antall funksjonelle stamfedre i en populasjon.
Tags
Quantitative AssayInsulin-expressing Colony-forming ProgenitorsPancreatic Stem CellsProgenitor Cell BiologyIn Vitro AssaySingle Cell AnalysisLineage PotentialPancreatospheresSuspension CultureSingle Cell DepositionCulture MediaNumerationLabor-intensivePancreatosphere-initiating ProgenitorsProliferation And DifferentiationSemi-solid Media Based Colony AssayMethylcelluloseThree-dimensional Space
