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Chapters
- 00:05Title
- 03:34Generating Conditioned Medium (CM) For the Colony Assay
- 05:13Obtaining a Single Cell Suspension of Pancreatic-like Progenitors by Fluorescence Activated Cell Sorting
- 07:55Plating Sorted Cells to Semi-solid Culture
- 10:49Observing Colony Formation under the Light Microscope
- 11:47Ngn3-EGFP+ Derived Colonies Express Insulin
- 13:21Conclusion
En tre-dimensionelle clonogenic analyse, der giver pancreas-lignende stamceller til at differentiere til insulin-udtrykkende kolonier er beskrevet. Denne metode benytter sig af semi-faste medier indeholdende methylcellulose, Matrigel og vækst faktorer, og hvor enkelt progenitorer formere sig og differentiere<em> In vitro</em>, Tillader kvantificering af antallet af funktionelle stamceller i en befolkning.
Tags
Quantitative AssayInsulin-expressing Colony-forming ProgenitorsPancreatic Stem CellsProgenitor Cell BiologyIn Vitro AssaySingle Cell AnalysisLineage PotentialPancreatospheresSuspension CultureSingle Cell DepositionCulture MediaNumerationLabor-intensivePancreatosphere-initiating ProgenitorsProliferation And DifferentiationSemi-solid Media Based Colony AssayMethylcelluloseThree-dimensional Space
