Journal
/
生物学
/
一个定量测定胰岛素表达细胞集落形成先觉
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
A Quantitative Assay for Insulin-expressing Colony-forming Progenitors
Please note that all translations are automatically generated. Click here for the English version.

一个定量测定胰岛素表达细胞集落形成先觉

DOI:
10.3791/3148-v

•

14:39 min

•

November 28, 2011

•

, , , , , ,
1Department of Biotechnology & Bioinformatics,California State University Channel Islands, 2Department of Diabetes, Endocrinology and Metabolism,Beckman Research Institute of City of Hope, 3The Irell & Manella Graduate School of Biological Sciences,Beckman Research Institute of City of Hope

Chapters

  • 00:05Title
  • 03:34Generating Conditioned Medium (CM) For the Colony Assay
  • 05:13Obtaining a Single Cell Suspension of Pancreatic-like Progenitors by Fluorescence Activated Cell Sorting
  • 07:55Plating Sorted Cells to Semi-solid Culture
  • 10:49Observing Colony Formation under the Light Microscope
  • 11:47Ngn3-EGFP+ Derived Colonies Express Insulin
  • 13:21Conclusion

Summary

自动翻译

自动翻译

描述一个立体的克隆形成实验，让胰祖细胞分化为胰岛素表达殖民地。此方法采用半固体甲基纤维素，基质胶和生长因子的媒体的优势，其中单祖细胞增殖和分化<em>在体外</em>，允许量化人口的功能祖。

Tags

Quantitative AssayInsulin-expressing Colony-forming ProgenitorsPancreatic Stem CellsProgenitor Cell BiologyIn Vitro AssaySingle Cell AnalysisLineage PotentialPancreatospheresSuspension CultureSingle Cell DepositionCulture MediaNumerationLabor-intensivePancreatosphere-initiating ProgenitorsProliferation And DifferentiationSemi-solid Media Based Colony AssayMethylcelluloseThree-dimensional Space

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/3148?article_type=v

脂肪酸定量磷酸化刺激酿酒酵母</em
check_url/cn/3148?article_type=v

成人内皮细胞集落形成祖细胞的分离和大型规模扩张
check_url/cn/3148?article_type=v

隔离和动物血清自由膨胀的人脐血源性间质基质细胞（MSCs），内皮细胞集落形成祖细胞（ECFCs）
check_url/cn/3148?article_type=v

使用CD71/TER119流流式细胞仪检测小鼠红系祖细胞的识别和分析
check_url/cn/3148?article_type=v

来自人类脐带血内皮细胞集落形成细胞的表型和功能特性
check_url/cn/3148?article_type=v

定量健身分析工作流程
check_url/cn/3148?article_type=v

高通量MHC II类分子结合分析抗原表位肽的定量分析
check_url/cn/3148?article_type=v

敏感，大规模定量代谢组学的策略
check_url/cn/3148?article_type=v

定量免疫检测，以衡量在中心体的变异蛋白水平
check_url/cn/3148?article_type=v

一个定量聚合酶重组酶扩增法的发展与内部阳性对照

Read Article