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Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice
Chapters
- 00:05Title
- 01:28Subcutaneous Administration of mAChR Antagonists
- 02:51Dissection of LAL Muscles
- 04:40Triple Immunostaining of LAL NMJs: Day 2
- 05:33The Effects of a Muscarinic Antagonist on NMJ Structure
- 06:49Conclusion
We describe procedures for repeated administration of inhibitors of muscarinic signaling to the levator auris longus (LAL) muscle of young adult mice and for subsequent immunostaining of its neuromuscular junctions (NMJs) in wholemounts. The LAL muscle has unique advantages for revealing in vivo pharmacological effects on NMJs.
Tags
Subcutaneous AdministrationMuscarinic AntagonistsTriple-immunostainingLevator Auris Longus MuscleMiceHind Limb MusclesIn Vivo Pharmacological StudiesNMJsDrug PenetrationSubcutaneous AdministrationIntramuscular AdministrationSystemic AdministrationMini-pumpsInflammatory ResponsesDirect Muscle DamageHind Limb Muscle AnalysisSerial SectioningExtensive ImmunostainingMouse LAL MusclePinna MovementFacial Nerve BranchStylomastoid Foramen
