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Medir a carga bacteriana e resposta imune em camundongos infectados com Listeria monocytogenes</em
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免疫与感染
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JoVE 杂志 免疫与感染
Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Medir a carga bacteriana e resposta imune em camundongos infectados com Listeria monocytogenes</em

30,562 Views

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12:50 min

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August 09, 2011

DOI:

10.3791/3076-v

DOI:
10.3791/3076-v

•

12:50 min
August 09, 2011

•

17 Views
, , ,
1St Vincent’s Institute, Department of Medicine,The University of Melbourne, 2Department of Microbiology and Immunology,The University of Melbourne

Summary

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Listeria monocytogenes é um organismo modelo para estudar as respostas imunológicas e suscetibilidade genética para as bactérias intracelulares em camundongos. Este método permite que sejam medidos carga bacteriana e gerar uma única célula suspensões de fígado e do baço de ratos para análise FACS para determinar mudanças em células do sistema imunológico devido à infecção por Listeria.

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Wang, N., Strugnell, R., Wijburg, O., Brodnicki, T. Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes. J. Vis. Exp. (54), e3076, doi:10.3791/3076 (2011).

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