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测量细菌感染小鼠和免疫反应李斯特菌</em
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
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测量细菌感染小鼠和免疫反应李斯特菌</em

30,560 Views

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12:50 min

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August 09, 2011

DOI:

10.3791/3076-v

DOI:
10.3791/3076-v

•

12:50 min
August 09, 2011

•

17 Views
, , ,
1St Vincent’s Institute, Department of Medicine,The University of Melbourne, 2Department of Microbiology and Immunology,The University of Melbourne

Summary

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李斯特菌是一种模式生物，为研究免疫反应和遗传易感性细胞在小鼠体内的细菌。这种方法使一个衡量细菌负荷，产生单细胞悬液，流式细胞仪分析小鼠肝和脾，以确定由于李斯特菌感染的免疫细胞的变化。

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Wang, N., Strugnell, R., Wijburg, O., Brodnicki, T. Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes. J. Vis. Exp. (54), e3076, doi:10.3791/3076 (2011).

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