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Lifetime Imaging di fluorescenza di rotori molecolari nelle cellule viventi
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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Lifetime Imaging di fluorescenza di rotori molecolari nelle cellule viventi

24,433 Views

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09:45 min

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February 09, 2012

DOI:

10.3791/2925-v

DOI:
10.3791/2925-v

•

09:45 min
February 09, 2012

•

4 Views
, , , ,
1Department of Physics,King’s College London, 2Department of Chemistry,Imperial College London , 3PhotoBiotics Ltd

Summary

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Lifetime Imaging di fluorescenza (FLIM) è emersa come una tecnica fondamentale per l'immagine l'ambiente e l'interazione di proteine ​​specifiche e coloranti nelle cellule viventi. FLIM fluorescenti di rotori molecolari consente mappatura di viscosità in cellule viventi.

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Cite this Article

Suhling, K., Levitt, J. A., Chung, P. H., Kuimova, M. K., Yahioglu, G. Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells. J. Vis. Exp. (60), e2925, doi:10.3791/2925 (2012).

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