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1Department of Medicine, David Geffen School of Medicine,University of California, Los Angeles, 2Research service, 151,Veterans Affairs Greater Los Angeles Healthcare System, 3Departments of Medicine, Urology at David Geffen School of Medicine and Department of Microbiology, Immunology and Molecular Gentics,University of California Los Angeles (UCLA), 4Division of Infectious Diseases, 111F,Veterans Affairs Greater Los Angeles Health Care System
Chapters
- 00:05Title
- 02:20Fixation of Leptospira to Glass Slides
- 04:51Labeling with Specific Antibodies
- 06:33Visualization of Leptospires
- 08:42Data Analysis and Representative Results
- 10:20Conclusion
Un método eficaz para evaluar la superficie de exposición de las proteínas de la leptospirosis se describe. El método está diseñado específicamente para evitar la interrupción de la frágil membrana externa de las células de leptospira. Esta técnica requiere el empleo de varios controles negativos para evaluar la integridad de la membrana externa y la especificidad de la reacción de los anticuerpos.
Tags
Immuno-fluorescence AssayLeptospiral Surface-exposed ProteinsBacterial Surface ProteinsCellular LocalizationFunctional RoleVirulence FactorsPathogenicity MechanismsFractionating Leptospiral MembranesOuter-membrane Proteins (OMPs)LipoproteinsTransmembrane OMPsHydrophobic InteractionLipid Bilayer PhospholipidsAmphipathic β-sheetsBarrel-like StructureOuter Membrane IntegrityImmunofluorescence Assay (IFA)
