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DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
JoVE 杂志
生物学
Author Produced
This content is Free Access.
JoVE 杂志 生物学
DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

DOI:
10.3791/2763-v

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13:32 min

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March 26, 2011

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, , ,
1Department of Integrative Oncology,BC Cancer Research Centre, 2Interdisciplinary Oncology Program,University of British Columbia – UBC, 3Photography/Video Production, Multi-Media Services,BC Cancer Agency, 4Department of Pathology and Laboratory Medicine,University of British Columbia – UBC

Chapters

  • 00:05Title
  • 00:38Paraffin Removal
  • 01:40Ethanol Rehydration
  • 02:58Tissue Digestion
  • 04:09DNA Clean Up
  • 07:12DNA Precipitation
  • 10:40DNA Quantification

Summary

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This video demonstrates the protocol for DNA extraction from formalin-fixed paraffin-embedded material. This is a multi-day procedure in which tissue sections are deparaffinized with xylene, rehydrated with ethanol and treated with proteinase K to purify and isolate DNA for subsequent gene-specific or genome-wide analysis.

Tags

DNA ExtractionParaffin Embedded MaterialGenetic AnalysisEpigenetic AnalysisChromosomal RearrangementsCopy Number Gains And LossesDNA MethylationHigh-throughput Profiling TechnologiesMicroarraysSample QualitySpecimen CollectionFormalin-fixed And Paraffin Embedded (FFPE)Hospital ArchivesExtraction MethodXylene TreatmentEthanol WashesProteinase KLysis Buffer

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