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从石蜡包埋材料的遗传和表观遗传分析的DNA提取

JoVE 杂志
生物学
Author Produced

This content is Free Access.

JoVE 杂志生物学

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

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从石蜡包埋材料的遗传和表观遗传分析的DNA提取

55,346 Views

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13:32 min

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March 26, 2011

DOI:

DOI:

•

13:32 min

March 26, 2011

•

28 Views

Larissa A. Pikor*^1,2, Katey S. S. Enfield*^1,2, Heryet Cameron³, Wan L. Lam^1,2,4

¹Department of Integrative Oncology,BC Cancer Research Centre, ²Interdisciplinary Oncology Program,University of British Columbia – UBC, ³Photography/Video Production, Multi-Media Services,BC Cancer Agency, ⁴Department of Pathology and Laboratory Medicine,University of British Columbia – UBC

Summary

本视频演示了从福尔马林固定石蜡包埋的材料DNA提取的协议。这是一个多天的过程中，水化组织切片用二甲苯deparaffinized，乙醇和蛋白酶K处理，净化和隔离随后的特定基因或全基因组分析的DNA。

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