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Modificato annessina V / propidio ioduro Apoptosis analisi di valutazione accurata di morte cellulare
JoVE 杂志
生物学
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JoVE 杂志 生物学
Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
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Modificato annessina V / propidio ioduro Apoptosis analisi di valutazione accurata di morte cellulare

101,443 Views

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07:34 min

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April 24, 2011

DOI:

10.3791/2597-v

DOI:
10.3791/2597-v

•

07:34 min
April 24, 2011

•

86 Views
, , ,
1Department of Biological Sciences,University of Alberta, 2Department of Agriculture, Food and Nutrition Sciences,University of Alberta

Summary

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Un metodo accurato per la valutazione della morte cellulare è descritto. Il protocollo migliora convenzionale annessina V / propidio ioduro (PI), i protocolli, che mostra fino al 40% di falsi positivi eventi in linee cellulari e cellule primarie da una vasta gamma di modelli animali.

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Cite this Article

Rieger, A. M., Nelson, K. L., Konowalchuk, J. D., Barreda, D. R. Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death. J. Vis. Exp. (50), e2597, doi:10.3791/2597 (2011).

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