Journal
/
生物学
/
改性膜联蛋白V /碘化丙啶检测细胞凋亡细胞死亡的准确评估
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
Please note that all translations are automatically generated. Click here for the English version.

改性膜联蛋白V /碘化丙啶检测细胞凋亡细胞死亡的准确评估

101,400 Views

•

07:34 min

•

April 24, 2011

DOI:

10.3791/2597-v

DOI:
10.3791/2597-v

•

07:34 min
April 24, 2011

•

86 Views
, , ,
1Department of Biological Sciences,University of Alberta, 2Department of Agriculture, Food and Nutrition Sciences,University of Alberta

Summary

Automatically generated

为评估细胞死亡的一种准确的方法是描述。该协议改进传统的膜联蛋白V /碘化丙碘化物（PI）的协议，这显示多达40％的假阳性的细胞株和原代细胞的动物模型的广泛事件。

Explore More Videos

Annexin V/Propidium Iodide Apoptosis AssayCell Death StudiesFlow Cytometry-based MethodsPlasma Membrane IntegrityPermeabilityCell ViabilityNuclear StainsLate Apoptotic CellsNecrotic CellsFalse Positive EventsRNA StainingCytoplasmic Compartment

Read Article

Cite this Article

Rieger, A. M., Nelson, K. L., Konowalchuk, J. D., Barreda, D. R. Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death. J. Vis. Exp. (50), e2597, doi:10.3791/2597 (2011).

Download .ris file

Copy

Share Video

.

Copy