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Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons
DOI:

09:54 min

April 06, 2011

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Chapters

  • 00:05Title
  • 00:57Introduction
  • 01:28Preparation and Maintenance of Sf9 Cell Monolayer Cultures
  • 03:35Expression of Fluorescently Tagged PKCs in Aplysia Sensory Neurons.
  • 06:16Visualization of PKC Translocation in Aplysia Sensory Neurons
  • 08:24Representative Results: Live-Imaging of PKC Translocation in Sf9 cells and in Aplysia Sensory Neurons
  • 09:29Conclusion

Summary

自动翻译

In this video, we demonstrate visualization of PKC translocation in living cells using fluorescently tagged PKCs.

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