Journal
/
神经科学
/
Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
JoVE 杂志
神经科学
Author Produced
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

DOI:
10.3791/2457-v

•

11:54 min

•

January 11, 2011

•

, , , ,
1Department of Anatomical Sciences,Shiraz University of Medical Sciences, Shiraz, Iran , 2Department of Neurosurgery,The University of Florida

Chapters

  • 00:01Title
  • 00:29Introduction
  • 00:57Animal Dissection
  • 02:40E14 Brain Dissection
  • 04:42Tissue Dissociation
  • 08:00Passaging Neurospheres
  • 10:10Representative Results
  • 11:31Conclusion

Summary

自动翻译

自动翻译

This video protocol demonstrates the application of the neurosphere assay for the isolation and expansion of neural stem cells from the ganglionic eminences of embryonic day 14-mouse brain.

Tags

Embryonic Mouse Neural Stem Cell CultureNeurosphere AssayStem CellsUndifferentiated CellsCell GenesisAging ProcessInjury And DiseaseNeural Stem Cells (NSCs)Serum-free Culture SystemIsolation And Expansion Of NSCsEmbryonic CNSIn Vitro Expanded NSCsCNS Cell TypesReliable And Consistent ResultsE14 Mouse EmbryosBrain MicrodissectionGanglionic EminencesSingle Cell SuspensionNSA CulturePrimary Neurospheres

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology

Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells

Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus

Den neuroblast Assay: Et assay for generering og berigelse af neuronale progenitorceller fra Differentiering neural stamcelle Afkom anvendelse af flowcytometri

Isolering og dyrkning af Rotte Embryonale Neurale Celler: En hurtig protokol

Isolering og dyrkning af neurale kamceller fra embryonale Murin Neural Tube

Kvantitativ måling af relative retinsyre Niveauer i E8.5 embryoner og neurosfære Cultures Brug af F9 RARE-LacZ Cell-baserede Reporter Assay

Tælling af neurale stamceller Brug Klonale Assays

Read Article