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Tillväxt och differentiering av vuxna hippocampus Arctic Neurala jordekorre Stamceller
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells
DOI:

09:44 min

January 07, 2011

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Chapters

  • 00:05Title
  • 01:03Thawing, Seeding, and Expanding Arctic Ground Squirrel Neural Stem Cells (agsNSC)
  • 01:05Preparing poly-L-ornithine-coated Flasks
  • 01:56Thawing and Seeding agsNSC
  • 03:39Expanding agsNSC
  • 04:11Trypsinization of Cells
  • 05:27Inoculating Well Plates for Differentiation
  • 07:25Maintenance of Neurons
  • 08:09Results of Growth and Differentiation of agsNSC
  • 09:29Conclusion

Summary

自动翻译

Neurala stamceller var beredda från hippocampus hos vuxna icke-ide ettåring arktiska marken ekorrar (AGS). Dessa neurala stamceller kan utökas genom många passager, differentierade och bevaras som ett nästan 50:50 neuron till gliaceller kultur.

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