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Chapters
- 00:00Title
- 01:05Introduction
- 02:28Harvestng Xenographs from Mice
- 03:22Generation of a Single-cell Suspension from Xenografts
- 04:12Removal of Tissue Debris and Dead Cells
- 05:44Staining Cells for Fluorescence Activated Cell Sorting (FACS)
- 07:43Isolation of Cancer Stem Cells by FACS
- 10:15Conclusion
Tags
IsolationStem CellsHumanPancreatic CancerXenograftsCSCsSelf-renewalDifferentiated ProgenyTumorImmunocompromised MiceBreast CancerCell Surface Antigen ExpressionCD44+CD24low/-ALDH ActivityPancreatic AdenocarcinomaCD133Tumorigenic PopulationsInvasive CellsProtocolViable Pancreatic CSCsLow-passage Human Xenografts
