This content is Free Access.
22,030 Views
•
11:44 min
•
September 26, 2010
DOI:
Summary
Automatically generated
癌症干细胞（CSCS）已确定在一些恶性肿瘤。在这个协议中，我们描述了一个流式细胞仪方法，利用醛脱氢酶活性和CD44和CD24的表达，从人胰腺癌移植瘤的社区体育会隔离。然后可以使用这些活细胞功能和分析研究。
Explore More Videos
IsolationStem CellsHumanPancreatic CancerXenograftsCSCsSelf-renewalDifferentiated ProgenyTumorImmunocompromised MiceBreast CancerCell Surface Antigen ExpressionCD44+CD24low/-ALDH ActivityPancreatic AdenocarcinomaCD133Tumorigenic PopulationsInvasive CellsProtocolViable Pancreatic CSCsLow-passage Human Xenografts
Read Article
Cite this Article
Rasheed, Z., Wang, Q., Matsui, W. Isolation of Stem Cells from Human Pancreatic Cancer Xenografts. J. Vis. Exp. (43), e2169, doi:10.3791/2169 (2010).
Copy