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蓝原生聚丙烯酰胺凝胶电泳（BN - PAGE）分析，从细胞裂解液多蛋白复合物
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12:03 min
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February 24, 2011
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在这个视频中，我们描述的多蛋白复合物（储值卡）蓝色原生聚丙烯酰胺凝胶电泳（BN - PAGE）的表征。在第一个层面，透析细胞裂解液的BN - PAGE分离，以确定个人的多用途储值卡。在第二维SDS - PAGE电泳，利益的储值卡是进一步细分免疫印迹分析其成分。
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Blue Native Polyacrylamide Gel ElectrophoresisBN-PAGEMultiprotein ComplexesCell SignallingProtein-protein Interaction NetworksNative ConditionsResolutionGel FiltrationSucrose Density UltracentrifugationMPC SizeCompositionRelative AbundanceHydrodynamic Size And ShapePolyacrylamide MatrixCellular LysatesLysate DialysisFirst Dimension BN-PAGESecond Dimension SDS-PAGE
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Fiala, G. J., Schamel, W. W. A., Blumenthal, B. Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates. J. Vis. Exp. (48), e2164, doi:10.3791/2164 (2011).
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