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Chapters
- 00:00Title
- 00:54Introduction
- 02:09Microdissection of Explants
- 07:16Microdissection and Culture of Central Explants
- 09:18Harvesting the Explants for Protein Analysis
- 10:19Harvesting the Explants for Immunofluorescence
- 12:15Immunofluorescence and Protein Analysis Results
- 15:54Conclusion
Explantaten van de centrale regio van de rat lens epitheel synchroon te onderscheiden wanneer gekweekt in de aanwezigheid van FGF-2. Immunofluorescentie microscopie van dergelijke culturen kan verschaft nieuwe informatie over de genexpressie en signalering afspraken verbonden aan terminale differentiatie.
Tags
PreparationCultureRat Lens Epithelial ExplantsTerminal DifferentiationAnterior SurfaceOcular LensEpithelial CellsProliferationAnnular ZoneCiliary BodyMigrationPosterior ArrayLens Fiber CellsBulk Of The LensIn Vitro InductionFGF-2Neonatal RatsLens CapsuleDissecting TweezersPeripheral RegionsCentral AreaFGF-2 ConcentrationTime CourseSignalingGene ExpressionMolecular TechniquesBiochemical TechniquesPharmacological TechniquesImmunofluorescence Microscopy
